Activation of caspases plays a central role in apoptosis. The Kit provides a convenient and sensitive means for detecting activated caspase-8 in situ in living cells. The assay utilizes the caspase-8 inhibitor IETD-FMK conjugated to FITC (FITC-IETD-FMK) as the ?uorescent in situ marker. FITC-IETD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-8 in apoptotic cells. The FITC label allows detection of activated caspase-8 in apoptotic cells directly by ?uorescence microscopy, ?ow cytometry or ?uorescence plate reader.
Kit Components: FITC-IETD-FMK 1x25ul
Wash Buffer 1x50ml