Synthetic peptide(KLH coupled) corresponding to N-terminal residues adjacent to Asp175 in human caspase-3. Synthetic peptide (KLH coupled) corresponding to residues surrounding the cleavage site and the amino terminus of the large fragment of human caspase-3.
Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases. Caspase-3 Antibody detects endogenous levels of full length caspase-3 (35kD) and the large fragment of caspase-3 resulting from cleavage (17kD).
Caspase-3 is one of the key executioners of apoptosis, being responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 subunits. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). Apoptosis Marker: Cleaved Caspase-3 (Asp175) Western Detection Kit contains enough primary caspase-3 and cleaved caspase-3 antibodies, and secondary anti-rabbit and anti-biotin antibodies to perform 10 Western mini-blots. The kit also comes with biotinylated molecular weight markers, a set of control cell lysates and reagent. The kit offers an efficient way of detecting caspase-3 processing and activation by Western immunoblotting.
Suitable for use in Western Blotting, Immunohistochemistry. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Purity: Purified by protein A and peptide affinity chromatography.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.