Caspases play a key role in apoptosis. Similar as other caspases, caspase-2 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Caspase-2 mRNA is alternatively spliced. The larger mRNA species encoding a product of 435aa is known as ICH-1L. The smaller mRNA species encoding a protein of 312aa is named ICH-1S. Over expression of ICH-1L induces apoptosis, while over expression of ICH-1S suppresses Rat-1 cell death induced by serum deprivation.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.