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CARMA1 (Bimp2, CARD11, Caspase Recruitment Domain-containing Protein 11, CARD-containing MAGUK Protein 1)

Cat no: C1385-26A

Supplier: United States Biological
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CARMA1 (Bimp3, CARD11) belongs to the MAGUK (membrane-associated guanylate kinase) family which typically function as molecular scaffolds in the assembly of multiprotein complexes (1, 2). MAGUK family members contain an SH3 domain, a PDZ domain and a GuK domain homologous to guanylate kinase. In addition, CARD11 contains an amino-terminal CARD domain (caspase recruitment domain). This domain plays an important role in forming interactions with a number of proteins containing CARD domains that are involved in regulating apoptosis and NF-kappa beta activation. CARD11 is predominately expressed in lymphocytes (1,2) and associates with the CARD domain of Bcl10. When overexpressed, CARD11 leads to the phosphorylation of Bcl10 and activation of NF-kappa beta (1, 2). CARD11 is constitutively associated with lipid rafts and is thought to function by recruiting Bcl10 and MALT1 and triggering the phosphorylation of IKKs (3, 4). Several studies using the genetic disruption of CARD11 or dominant-negative mutations have demonstrated that it plays a critical role in NF-kappa beta activation and lymphocyte signaling (4-7). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted C1385-26A in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C1385-26A
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Bertin, J. et al. (2001) J. Biol. Chem. 276, 11877-11882. 2. Gaide, O. et al. (2001) FEBS Lett. 496, 121-127. 3. Stilo, R. et al. (2004) J. Biol. Chem. 279, 34323-34331. 4. Wang, D. et al. (2002) Nat. Immunol. 3, 830-835. 5. Jun, J.E. et al. (2003) Immunity 18, 751-762. 6. Hara, H. et al. (2003) Immunity 18, 763-775. 7. Gaide, O. et al. (2002) Nat. Immunol. 3, 836-843.
Additional info: Recognizes endogenous levels of total CARMA1 protein at ~130kD. Species Crossreactivity: Human, mouse Species Sequence Homology: Rat, monkey

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