Ca(2+)/calmodulin-dependent protein kinase IV (CaMKIV) is a multifunctional protein kinase expressed abundantly in the central nervous system. Because changes in intracellular Ca(2+) concentrations affect progression through the mitotic cell cycle, enhanced expression of
CaMKIV has been reported in small cell lung carcinoma and hepatocellular carcinoma (1). Multifunctional Ca2+/calmodulin kinase IV is enriched in neurons and T lymphocytes. The kinase is absent from primary human B lymphocytes but is expressed in Epstein-Barr virus (EBV)- transformed B-lymphoblastoid cell lines, suggesting that expression of the kinase can be upregulated by an EBV gene products. In view of the key role played by LMP1 in B- lymphocyte immortalization by EBV, findings implicate Ca2+/calmodulin kinase IV as a potential mediator of B- lymphocyte growth transformation (2). Ca2+/calmodulin kinase IV can suppress the MT-regulating activity of Op18 by phosphorylation on Ser-16. These results show that Op18
phosphorylation by Ca2+/calmodulin kinase IV may couple alterations of MT dynamics in response to external signals that involve Ca2+ (3).
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:60
Western Blot: 1:100,000-1:500,000
Immunoprecipitation: 1:60
Immunohistochemistry: 1:250-1:500
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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