Total c-Jun Sandwich ELISA Antibody Pair is being offered as an alternative to our Total c-Jun Sandwich ELISA Kit. Capture and Detection antibodies (100X stocks) and a HRP-Conjugated Secondary Antibody (1000X stock) is supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The c-Jun Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by c-Jun Detection Antibody and HRP-Conjugated Secondary Antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of total c-Jun protein.
A. Kit Components:
Total c-Jun Capture Antibody (100X), 1x400ul
Total c-Jun Detection Antibody (100X), 1x4000ul
Anti-mouse IgG (HRP) (1000X), 1x40ul
B. Reagents Required But Not Supplied:
1. Coating Buffer: 1X PBS, (20X PBS) 3.2 mM Na 2HPO4, 0.5 mM KH2PO4, 1.3 mM KCl, 135 mM NaCl, pH 7.4
2. Wash Buffer: 1X PBS/0.05% Tween-20, (20X PBST)
3. Blocking Buffer: 1X PBS/0.05% Tween-20, 1% BSA
4. 1X Cell Lysis Buffer: (10X Cell Lysis Buffer) 20 mM Tris (pH 7.5), 150 mM NaCl, 1 mM ethylene diamine tetraacetate (EDTA), 1 mM ethylene glycol-bis(2-aminoethyl)-N,N,N
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