Herstatin is a secreted 68kD glycoprotein encoded by an alternate transcript of the HER-2/neu (ErbB-2) gene containing the N-terminal 304aa of subdomain I and II, and 79 intron-encoded aa at the C-terminus. It is an endogenous inhibitor of the EGF receptor family that disrupts receptor interactions and inhibits growth of tumor cells that overexpress HER-2.
Suitable for use in Immunohistochemistry. Other applications not tested.
Immunohistochemistry: 25ug/ml detects c-erbB2 in paraffin-embedded human breast cancer tissue sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.