c-Cbl, product of c-cbl proto-oncogene, is a 120kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4). It has been demonstrated that certain tyrosines are the major phosphorylation sites of c-Cbl in T cells in response to pervanadate treatment, as well as in response to TcR/CD3 ligation. Cbl also enhances migration of v-Abl-transformed NIH 3T3 fibroblasts. This effect of Cbl depends on its tyrosine phosphorylation, which is required for binding of PI-3' kinase to c-Cbl (5)
Applications:
Suitable for use inFlow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000
Immunocytochemistry: 1:100-1:250
Flow Cytometry: 1:20
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.