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Btk, CT, phosphorylated, Tyr551 (Bruton

Cat no: B5000-01A

Supplier: United States Biological
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Btk (Bruton tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. Btk is composed of a pleckstrin domain, an SH3 and SH2 domain, a catalytic kinase domain and a TEC homology domain (1). BTK plays a crucial role in B cell activation and development (2). Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner (3) Btk can be activated through intramolecular autophosphorylation at tyrosine 551 and that Btk autophosphorylation is required for Btk catalytic functions (4). Mutations in the Btk gene have been linked to severe developmental blocks in human B-cell ontogeny leading to X-linked agammaglobulinemia (XLA). Patients with this disease are unusually prone to bacterial infection but not to viral infection (5). Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000. Immunocytochemistry: 1:100-250. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: B5000-01A
Reactivities: Human
Hosts: Rabbit
Applications: Immunocytochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Tsukada, S., Saffaran, D., Rawlings, D. & Witte, O. (1993) Cell 72, 279-290 2. Rawlings, D. J. & Witte, O. N. (1995) Semin. Immunol. 7, 237-246 3. Vassilev, A., Z. Ozer, C. Navara, S. Mahajan, and F. M. Uckun. 1999. J. Biol. Chem. 274:1646-1656 4. Mahajan S, Fargnoli J, Burkhardt A L, Kut S A, Saouaf S J, Bolen J B. Mol Cell Biol. 1995;15:5304
Additional info: Recognizes human Btk, MW74kD, when phoshorylated at Tyr551.

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