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Bcl-2 (B Cell Leukemia 2) (PE)

Cat no: B0807-06H

Supplier: United States Biological
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The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Western Blot: Unconjugated Optimal dilutions to be determined by the researcher.. Flow Cytometry Protocol: Incubations and washes using a buffer containing 0.5% Tween-20 to permeabolize cell, were done at RT. 1. Cultured Nalm-6 human tumor cells were fixed for 20 min with 0.5% freshly prepared buffered formaldehyde after which they were washed two times. 2. Five x 10e5 cells per tube were incubated 45 minutes with 80ul of B0807-06H at a 1:50 dilution.
Catalogue number: B0807-06H
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Western Blot
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. F. Pezzella, et al, (1990) Am J Pathol 137: 225-232. I.A. Doussis, et al, (1993) Am J Clin Pathol 99: 663-667. 2. S. Cory, (1995) Annu Rev Immunol 13: 513-543.
Additional info: Recognizes the intracellular 26kD of the human Bcl-2 protein.

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