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B-Raf Kinase Assay Kit, Chemiluminescence, BioAssay(TM) (BRAF, Ras effector)

B-Raf Kinase Assay Kit, Chemiluminescence, BioAssay(TM) (BRAF, Ras effector)

Cat no: B2680-02A

Supplier: United States Biological
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B-Raf Kinase Assay Kit is designed to measure B-Raf dependent phosphotransferase activity in a kinase reaction using recombinant MEK1, unactive as a B-Raf substrate. The enzyme assay is rapid, convenient and specific for the Raf family of kinases. 50 kinase assays per kit. B-Raf Kinase Cascade Reaction: MEK1, unactive + ATP ----- Active B-Raf, 30 degrees C, 30 min ----> Active MEK1 + ADP Kit Components: B2680-02A1: B-Raf (delta1-415), active, Recombinant 1x10ug B2680-02A2: MEK1 (human), unactive, Recombinant 1x50ug B2680-02A3: Assay Dilution Buffer I (ADBI) 1x1ml B2680-02A4: Magnesium/ATP Cocktail 1x1ml B2680-02A5: MEK1 (Ser218/222) / MEK2 (Ser222/226) phosphorylated 1x200ug Storage and Stability: Store components at -70 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B2680-02A
Size: 1Kit
References: US Biological application reference: Robinson-White, A.J. et al., (2006) Cancer Research 66:10603-10612. 1. Alessi. D.R., et al., Methods Enzymol. 255: 279-289 (1995). 2. Alessi, D.R., et al., EMBO J. 13: 1610-1619 (1994). 3. Van Aelst, L., et al., PNAS USA 90: 6213-6217 (1993). 4. Jelinek, T., et al., Mol. Cell. Biol. 14: 8212-8218 (1994). 5. Rossomando, A.J., et al., Mol. Cell. Biol. 14: 1594-1602 (1994). 6. Reuter, C.W., et al., J. Biol. Chem. 270: 7645-7655 (1995). 7. Barnier, J.V., et al., J. Biol. Chem. 270: 23,381-23,389 (1995).

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