The United States Biological Human beta Amyloid 1-40 (Hu A beta40) ELISA kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for the NH2-terminus of Hu b-amyloid has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known Hu b-amyloid 40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-40 b-amyloid sequence. This COOH- terminal sequence is created upon cleavage of the analyzed precursor. Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody. After washing, horseradish peroxidase-labeled anti-rabbit antibody (enzyme) is added. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu A beta40 present in the original specimen.
Sensitivity:
<6pg/ml
Range:
7.8-500pg/ml
Kit Components:
*A2275-73M1: beta Amyloid 1-40 Standard, 1x1vial
A2275-73M2: Standard Diluent Buffer, 1x60ml
A2275-73M3: Microtiter Plate, 1x96 wells
A2275-73M4: beta Amyloid 1-40, Pab 1x6ml
A2275-73M5: IgG (HRP) (100X), 1x125ul
A2275-73M6: HRP Diluent, 1x25ml
A2275-73M7: Wash Buffer (25X), 1x100ml
A2275-73M8: TMB, 1x25ml
A2275-73M9: Stop Solution, 1x25ml
Storage and Stability:
Store *A2275-73M1 powder at 4 degrees C liquid at -70 degrees C. Store other components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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