DDC (EC 1.1.128) belongs to the family group II Decarboxylase (DDC, GAD, HDC and TYRD). DDC catalyzes the formation of tryptamine from L-tyrosine. It also acts on 5-hydroxy-LO-tryptophan and dihydroxy-L-Phenylalanine (DOPA). Human DDC (480aa, chromosome 7p11) is a homodimer in the native state. it requires pyridoxal phosphate as a cofactor.
A 16 amino acid peptide sequence near the N-terminus of human DDC.
ELISA: Use at 1ug/ml to coat plates and detect with antibodies (1;10,000-1:50,000).
Note: Not suitable for Western Blot due to low MW (< 3kD).
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.