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Apoptosis, Human BioAssay(TM) Sample Kit

Cat no: A1059-89

Supplier: United States Biological
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Apoptosis, or programmed cell death, is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 8, 9, 10 and 12) are closely coupled to proapoptotic signals. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, alpha-Fodrin, DFF and Lamin A, and induce apoptosis. Cytochrome c released from mitochondria is coupled to the activation of caspase-9, a key initiator caspase. Proapoptotic stimuli include the FasL, TNF-alpha, DNA damage and ER stress. Fas and TNFR activate caspases 8 and 10; DNA damage leads to the activation of caspase-9; and ER stress leads to the calcium-mediated activation of caspase-12. The inhibitor of apoptosis protein (IAP) family includes XIAP and Survivin and functions by binding and inhibiting several caspases (4,5). Smac/Diablo, a mitochondrial protein, is released into the cytosol upon mitochondrial stress and competes with caspases for binding of IAPs. The interaction of Smac/Diablo with IAPs relieves the inhibitory effects of the IAPs on caspases (6).
Catalogue number: A1059-89
Size: 8x40ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Purity: Purified by protein A and peptide affinity chromatography.
References: (1) Baker, S.J. and Reddy, E.P. (1998) Oncogene 17, 3261
Additional info: Each antibody in the Apoptosis Sampler Kit detects endogenous levels of its respective protein. Cleaved Caspase-3 (Asp175), Cleaved Caspase-7 (Asp198) and Cleaved PARP (Asp214) Antibodies recognize only the large fragments of their respective cleaved proteins. Cleaved Caspase-9 (Asp330) Antibody recognizes either the 37kD (with prodomain) or 17kD large fragment of the cleaved protein. Caspase-3, Caspase-7, Caspase-9 and PARP Antibodies recognize both the full length and large cleaved fragments of their respective proteins.

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