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Apolipoprotein B (Apo B, APOB, Apolipoprotein B-100, Apo B-100)

Cat no: A2299-46H

Supplier: United States Biological
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Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549kD and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/L. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 5-10ug/ml Immunohistochemistry: 5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A2299-46H
Reactivities: Human
Hosts: Goat
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.01% thimerosal, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
Additional info: Recognizes human ApoLipoprotein. It shows negligible crossreactivity with type A-I, A-II, C-I, C-II, C-III, E, and J apoLipoproteins (< 1%).

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