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Apolipoprotein A-I (Apo A-I)

Apolipoprotein A-I (Apo A-I)

Cat no: A2299-30

Supplier: United States Biological
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Preparation of Protein: Apo AI can be renatured into PBS, TBS, or other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14,000 or less. This should be performed in a cold room. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Storage of dialyzed Apo AI in PBS or TBS, 1mM EDTA, 0.1% sodium azide should be stored (up to 2 weeks) at 4 degrees C. Storage of Apo AI in PBS or TBS at -20 degrees C may lead to precipitation when thawed.
Catalogue number: A2299-30
Size: 1mg
Form: Supplied as a liquid, in 3M guanidine hydrochloride, 10mM Tris, 5mM DTT, pH 8.0. No preservative added.
Purity: (same/more than)99% by SDS-PAGE, purified from high density lipoprotein (HDL) from pooled human plasma. The protein was isolated by ultracentrifugal flotations, lyophilized then subjected to repeated ethanol extraction. The precipitated protein was dried under nitrogen then dissolved in 6M guanidine hydrochloride. The product was then subjected to Sephacryl S200 filtration and eluted then stored in 3M guanidine hydrochloride.
References: 1.Chen, W., et al., (2001), The Journal of Biological Chemistry, 276(47): 43564-43569 2.Feng, B., et al., (2002), The Journal of Biological Chemistry, 277(45): 43271-43280 3.Gerbod-Giannone, M.C., et al., (2006), PNAS, 103(9): 3112-3117 4.Martinez, L.O., (2003), The Journal of Biological Chemistry, 278(39): 37368-37374 5.Sun, Y., et al., (2003), The Journal of Biological Chemistry, 278(8): 5813-5820 6. Ishiguro, H., et al., (2001), The Journal of Biological Chemistry, 276(39): 36742-36748.

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