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Aldo-Keto reductase family1, member B1, 1-316aa, Recombinant, Human

Cat no: A1334-70A

Supplier: United States Biological
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Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Source: Human Aldose reductase (AKR1B1) protein was expressed in E. coli and purified by using conventional chromatography techniques. Specific Activity: 0.5-0.6 units/mg protein. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25 degrees C. Activity Assay: 1. Prepare a 750ul reaction mix into a suitable container. The final concentrations of the reaction mix components should be: 0.1M sodium phosphate (pH7.0) and 0.3mM NADPH. 2. Add 50ul of recombinant AKR1B1 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25 degrees C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde substrate and immediately mix by inversion. 5. Record the increase in A340nm for 3 minutes. AA Sequence: MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF Endotoxin level: (same/less than)1.0 EU per 1 ug of protein (LAL) Storage and Stability: For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A1334-70A
Size: 100ug
Form: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 10% glycerol.
Purity: (same/more than) 95% by SDS-PAGE
Alternative names: AKR1B1, ADR, ALDR1, ALR2, AR
References: 1. Ruiz FX., et al: (2009) Chem Biol Interact.178(1-3) 2. Donaqhue KC., et al: Diabet Med. 22(10):1315-20

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