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Adiponectin, Human BioAssay(TM) ELISA Kit

Cat no: A0725-53

Supplier: United States Biological
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In BioAssay(TM) Human Adiponectin ELISA, standards, quality control and samples of sera (or plasma) are incubated in microtitration wells coated with recombinant human adiponectin together with horseradish peroxidase-labeled anti-adiponectin antibody (conjugate). After a thorough wash, the conjugate bound to the adiponectin-coated wells is allowed to react with the hydrogen peroxide/TMB substrate solution. The reaction is stopped by the addition of sulfuric acid solution and the absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is inversely proportional to the adiponectin concentration. A standard curve is constructed by plotting the absorbance values versus the concentrations of serum adiponectin standards. The concentrations of unknown samples are determined (ug/ml) using this standard curve. Kit Components: A0725-53A: Microtiter Strips, 1x96 wells A0725-53B: Conjugate Solution, 1x7ml A0725-53C:10ug/ml Standard, 1x1vial A0725-53D: 5ug/ml Standard, 1x1vial A0725-53E: 2ug/ml Standard, 1x1vial A0725-53F: 1ug/ml Standard, 1x1vial A0725-53G: 0.5ug/ml Standard, 1x1vial A0725-53H: 0.2ug/ml Standard, 1x1vial A0725-53J: 0.1ug/ml Standard, 1x1vial A0725-53K: High Control, 1x400ul A0725-53L: Low Control, 1x400ul A0725-53M: Dilution Buffer, 2x13ml A0725-53N: Wash Solution Concentrate (10x), 1x100ml A0725-53P: Substrate (TMB) Solution, 2x13ml A0725-53Q: Stop Solution, H2SO4, 1x9ml Storage and Stability: Store all components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial and prior to removing the cap.
Catalogue number: A0725-53
Size: 1Kit
References: US Biological application reference: Pino, A.M. (2010) J. Bone and Mineral Research 25:492-498. 1. Scherer PE, Williams S, Fogliano M, Baldini G, Lodish HF. A novel serum protein similar to C1q, produced exclusively in adipocytes. J Biol Chem. 1995, 270, 26746-26749. 2. Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, Matsubara K. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (adipose most abundant gene transcript 1). Biochem Biophys Res Commun. 1996, 221, 286-289. 3. Arita Y, Kihara S, Ouchi N, Takahashi M, Maeda K, Miyagawa J, Hotta K, Shimomura I, Nakamura T, Miyaoka K, Kuriyama H, Nishida M, Yamashita S, Okubo K, Matsubara K, Muraguchi M, Ohmoto Y, Funahashi T, Matsuzawa Y. Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity. Biochem Biophys Res Commun. 1999, 257, 79-83. 4. Saito K, Tobe T, Minoshima S, Asakawa S, Sumiya J, Yoda M, Nakano Y, Shimizu N, Tomita M. Organization of the gene for gelatin-binding protein (GBP28). Gene. 1999, 229, 67-73. 5. Yokota T, Oritani K, Takahashi I, Ishikawa J, Matsuyama A, Ouchi N, Kihara S, Funahashi T, Tenner AJ, Tomiyama Y, Matsuzawa Y. Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages. Blood. 2000, 96, 1723-1732. 6. Das K, Lin Y, Widen E, Zhang Y, Scherer PE. Chromosomal localization, expression pattern, and promoter analysis of the mouse gene encoding adipocyte-specific secretory protein Acrp30. Biochem Biophys Res Commun. 2001, 280, 1120-1129. 7. Tsao TS, Lodish HF, Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Eur J Pharmacol. 2002, 440, 213-21. 8. Wang Y, Xu A, Knight C, Xu LY, Cooper GJ. Hydroxylation and glycosylation of the four conserved lysine residues in the collagenous domain of adiponectin. Potential role in the modulation of its insulin-sensitizing activity. J Biol Chem. 2002, 277, 19521-19529. 9. Kondo H, Shimomura I, Matsukawa Y, Kumada M, Takahashi M, Matsuda M, Ouchi N, Kihara S, Kawamoto T, Sumitsuji S, Funahashi T, Matsuzawa Y. Association of adiponectin mutation with type 2 diabetes: a candidate gene for the insulin resistance syndrome. Diabetes. 2002, 51, 2325-2328. 10. Spranger J, Kroke A, Mohlig M, Bergmann MM, Ristow M, Boeing H, Pfeiffer AF. Adiponectin and protection against type 2 diabetes mellitus. Lancet. 2003, 361, 226-228. 11. Pajvani UB, Du X, Combs TP, Berg AH, Rajala MW, Schulthess T, Engel J, Brownlee M, Scherer PE. Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications for metabolic regulation and bioactivity. J Biol Chem. 2003, 278, 9073-9085. 12. Daimon M, Oizumi T, Saitoh T, Kameda W, Hirata A, Yamaguchi H, Ohnuma H, Igarashi M, Tominaga M, Kato T. Decreased serum levels of adiponectin are a risk factor for the progression to type 2 diabetes in the Japanese Population: the Funagata study. Diabetes Care. 2003 Jul;26(7):2015-20. 13. Yamauchi T, Kamon J, Waki H, Imai Y, Shimozawa N, Hioki K, Uchida S, Ito Y, Takakuwa K, Matsui J, Takata M, Eto K, Terauchi Y, Komeda K, Tsunoda M, Murakami K, Ohnishi Y, Naitoh T, Yamamura K, Ueyama Y, Froguel P, Kimura S, Nagai R, Kadowaki T. Globular adiponectin protected ob/ob mice from diabetes and ApoE-deficient mice from atherosclerosis. J Biol Chem. 2003, 278, 2461-2468. 14. Yamauchi T, Kamon J, Ito Y, Tsuchida A, Yokomizo T, Kita S, Sugiyama T, Miyagishi M, Hara K, Tsunoda M, Murakami K, Ohteki T, Uchida S, Takekawa S, Waki H, Tsuno NH, Shibata Y, Terauchi Y, Froguel P, Tobe K, Koyasu S, Taira K, Kitamura T, Shimizu T, Nagai R, Kadowaki T. Cloning of adiponectin receptors that mediate antidiabetic metabolic effects. Nature. 2003, 423, 762-769.

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