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5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)

5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)

Cat no: N7100-71A

Supplier: United States Biological
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CD73 (Ecto-5'nucleotidase, 5?'-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:1000 Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Hybridoma: Mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD73 protein. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: N7100-71A
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Western Blot
Size: 50ul
Form: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
P type: Mab
Isotype: IgG2b,k
Purity: Purified by Protein G affinity chromatography.
References: 1.Sern P.C, et al., (2006) Purinergic Signal 2(2):351-360 2. Linda F.T, et al., (2004) J Exp Med 200(11):1395-1405 3. Laura A, et al., (1997) J Cell Biol 136(2):421-431
Additional info: Recognizes human 5'-Nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT).

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