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4-1BB Ligand (4-1BBL) (PE)

Cat no: 0004-01G


Supplier: United States Biological
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4-1BB Ligand (4-1BBL) is a type II transmembrane glycoprotein belonging to TNF superfamily (TNFSF) and has been designated TNFSF9. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C-terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that other TNFSF members, but shares only low aa sequence homology (14-16%). Murine 4-1BBL shares 36% aa sequence identity with its human counterpart.1, 2 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines.3 A soluble 4-1BBL is released from the cell surface following cellular activation via proteolytic cleavage by one or more sheddases.4 By analogy to other TNFSF ligands, both the soluble and transmembrane 4-1BBL are expected to exist as non-covalent homotrimers. 4-1BBL binds 4-1BB, a TNF receptor superfamily member, TNFRSF9, which is also known as CD137 and ILA (induced by lymphocyte activation). 4-1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils. In response to 4-1BBL binding, 4-1BB transduces a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In dendritic cells, 4-1BB is a DC-activating molecules that enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules.3 Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 0004-01G
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with PE.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G affinity chromatography.
References: 1. Goodwin, R.G. et al., 1993, Eur. J. Immunol. 23:2631. 2. Alderson, M.R. et al., 1994, Eur. J. Immunol. 24:2219. 3. Kwon, B., H.W. Lee and B.S Kwon, 2002, TRENDS in Immunology 23:378. 4. Salih, H.R. et al., 2001, J. Immunol. 167:4059.
Additional info: Recognizes mouse 4-1BB Ligand/TNFSF9.

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